Density Gradient Centrifugation

Density Gradient Centrifugation (also known as Ficoll-Paque¹, Pancoll², or Lymphoprep³) is the most common method for separating PBMC (perioheral blood mononuclear cells - containing lymphocytes and monocytes) from red blood cells (RBC, erythrocytes). Based on different density of each blood cell population they can be separated by a single step centrifugation with the corresponding density gradient medium. Beside of the most common solution for the isolation of PBMCs (density 1.077 g/ml) pluriSelect offers optimized density gradient media for the isolation of Leukocytes, Monocytes, Platelets and PBMCs from older blood. Furthermore pluriMate® and TwinSpin® centrifugation tubes simplify handling and accelerate purification.

¹Ficoll-Paque is a trademark of GE Healthcare/Cytiva
²Pancoll is a trademark of PAN-Biotech GmbH
³Lymphoprep is a trademark of Alere Technologies